Advanced Photon Counting

Applications, Methods, Instrumentation

Nonfiction, Science & Nature, Technology, Lasers, Science, Physics, Spectrum Analysis
Cover of the book Advanced Photon Counting by , Springer International Publishing
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: ISBN: 9783319156361
Publisher: Springer International Publishing Publication: April 23, 2015
Imprint: Springer Language: English
Author:
ISBN: 9783319156361
Publisher: Springer International Publishing
Publication: April 23, 2015
Imprint: Springer
Language: English

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

More books from Springer International Publishing

Cover of the book Educational Media and Technology Yearbook by
Cover of the book Machine Learning for Evolution Strategies by
Cover of the book INT-Integrated Neurocognitive Therapy for Schizophrenia Patients by
Cover of the book Pancreatic Masses by
Cover of the book Evolution of Smart Grids by
Cover of the book Motion Correction in Thoracic Positron Emission Tomography by
Cover of the book Decision Making and Knowledge Decision Support Systems by
Cover of the book Scars and Wounds by
Cover of the book State of the Art in Digital Media and Applications by
Cover of the book Out-of-order Parallel Discrete Event Simulation for Electronic System-level Design by
Cover of the book Computational Color Imaging by
Cover of the book Preschool Bilingual Education by
Cover of the book Ecological Processes at Marine Fronts by
Cover of the book Digital Breast Tomosynthesis by
Cover of the book Nanovate by
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy