Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA

Nonfiction, Science & Nature, Science, Chemistry, Organic, Technical & Industrial
Cover of the book Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA by Naohiro Terasaka, Springer Japan
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Naohiro Terasaka ISBN: 9784431565178
Publisher: Springer Japan Publication: March 8, 2017
Imprint: Springer Language: English
Author: Naohiro Terasaka
ISBN: 9784431565178
Publisher: Springer Japan
Publication: March 8, 2017
Imprint: Springer
Language: English

In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery.
The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery.
The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers.

More books from Springer Japan

Cover of the book Nitric Oxide in the Eye by Naohiro Terasaka
Cover of the book MRI of the Central Nervous System by Naohiro Terasaka
Cover of the book Biological Effects by Organotins by Naohiro Terasaka
Cover of the book Zoom Gastroscopy by Naohiro Terasaka
Cover of the book Schwann Cell Development and Pathology by Naohiro Terasaka
Cover of the book Livelihood Security in Northwestern Himalaya by Naohiro Terasaka
Cover of the book Post-Genome Biology of Primates by Naohiro Terasaka
Cover of the book Anomalous and Topological Hall Effects in Itinerant Magnets by Naohiro Terasaka
Cover of the book Application of Recirculating Aquaculture Systems in Japan by Naohiro Terasaka
Cover of the book Novel Insights in Agent-based Complex Automated Negotiation by Naohiro Terasaka
Cover of the book Operative General Surgery in Neonates and Infants by Naohiro Terasaka
Cover of the book Economic Effects of Public Investment by Naohiro Terasaka
Cover of the book Morpho-functional Machines: The New Species by Naohiro Terasaka
Cover of the book Social-Ecological Systems in Transition by Naohiro Terasaka
Cover of the book Dynamics of Learning in Neanderthals and Modern Humans Volume 1 by Naohiro Terasaka
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy