Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis

Nonfiction, Science & Nature, Science, Chemistry, Analytic, Biological Sciences, Biochemistry
Cover of the book Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis by Fangjun Wang, Springer Berlin Heidelberg
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Fangjun Wang ISBN: 9783642420085
Publisher: Springer Berlin Heidelberg Publication: December 2, 2013
Imprint: Springer Language: English
Author: Fangjun Wang
ISBN: 9783642420085
Publisher: Springer Berlin Heidelberg
Publication: December 2, 2013
Imprint: Springer
Language: English

In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer’s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research.

More books from Springer Berlin Heidelberg

Cover of the book Studying Cell Metabolism and Cell Interactions Using Microfluidic Devices Coupled with Mass Spectrometry by Fangjun Wang
Cover of the book Mucosal Vaccines by Fangjun Wang
Cover of the book Advanced Models of Neural Networks by Fangjun Wang
Cover of the book CSR und Tourismus by Fangjun Wang
Cover of the book Diabetes - Perspectives in Drug Therapy by Fangjun Wang
Cover of the book Der Experimentator Molekularbiologie / Genomics by Fangjun Wang
Cover of the book The Chemokine System in Experimental and Clinical Hematology by Fangjun Wang
Cover of the book Indirect and Direct Wallerian Degeneration in the Intramedullary Root Fibres of the Hypoglossal Nerve by Fangjun Wang
Cover of the book Political Ethics and European Constitution by Fangjun Wang
Cover of the book Studying Vibrational Communication by Fangjun Wang
Cover of the book Molecular Conformation and Organic Photochemistry by Fangjun Wang
Cover of the book The Road to Privatization of Higher Education in China by Fangjun Wang
Cover of the book Motion Vision by Fangjun Wang
Cover of the book Gynecology and Obstetrics Urology by Fangjun Wang
Cover of the book Mathematik by Fangjun Wang
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy