Directed Evolution of Selective Enzymes

Catalysts for Organic Chemistry and Biotechnology

Nonfiction, Science & Nature, Science, Chemistry, Technical & Industrial
Cover of the book Directed Evolution of Selective Enzymes by Manfred T. Reetz, Wiley
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Author: Manfred T. Reetz ISBN: 9783527655489
Publisher: Wiley Publication: September 7, 2016
Imprint: Wiley-VCH Language: English
Author: Manfred T. Reetz
ISBN: 9783527655489
Publisher: Wiley
Publication: September 7, 2016
Imprint: Wiley-VCH
Language: English

Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods.
Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments.

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Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods.
Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments.

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