Enzymatic Analysis

A Practical Guide

Nonfiction, Science & Nature, Science, Biological Sciences, Biochemistry
Cover of the book Enzymatic Analysis by Janet V. Passonneau, Oliver H. Lowry, Humana Press
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Janet V. Passonneau, Oliver H. Lowry ISBN: 9781603274074
Publisher: Humana Press Publication: February 7, 2008
Imprint: Humana Language: English
Author: Janet V. Passonneau, Oliver H. Lowry
ISBN: 9781603274074
Publisher: Humana Press
Publication: February 7, 2008
Imprint: Humana
Language: English

Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.

More books from Humana Press

Cover of the book Male Infertility by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Heart Failure by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Stem Cells & Regenerative Medicine by Janet V. Passonneau, Oliver H. Lowry
Cover of the book An Internist’s Illustrated Guide to Gastrointestinal Surgery by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Studies on Experimental Models by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Quiet Lives by Janet V. Passonneau, Oliver H. Lowry
Cover of the book The Nucleic Acid Protocols Handbook by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Microbiology and Aging by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Beverages in Nutrition and Health by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Age-Related Changes of the Human Eye by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Drug Development by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Principles and Practice of Cancer Infectious Diseases by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Handbook of Blood Pressure Measurement by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Essentials of Sleep Medicine by Janet V. Passonneau, Oliver H. Lowry
Cover of the book Oxidative Stress and Free Radical Damage in Neurology by Janet V. Passonneau, Oliver H. Lowry
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy