Rapid Cycle Real-Time PCR — Methods and Applications

Genetics and Oncology

Nonfiction, Science & Nature, Science, Other Sciences, Molecular Biology, Health & Well Being, Medical, Specialties, Oncology
Cover of the book Rapid Cycle Real-Time PCR — Methods and Applications by , Springer Berlin Heidelberg
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Author: ISBN: 9783642593970
Publisher: Springer Berlin Heidelberg Publication: June 29, 2013
Imprint: Springer Language: English
Author:
ISBN: 9783642593970
Publisher: Springer Berlin Heidelberg
Publication: June 29, 2013
Imprint: Springer
Language: English

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

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Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

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